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建立了光度法测定硫普罗宁的新方法。研究表明:在SCN-和KNO3存在下,控制溶液pH4.0,Cu(II)被硫普罗宁还原生成的Cu(I)与SCN-反应形成CuSCN沉淀,该沉淀能浮在水相表面。通过测定溶液中剩余Cu(II)的量,可以测定硫普罗宁的含量。吸光度与硫普罗宁浓度之间存在良好线性关系。线性方程:A=4.898-0.3616ρ(μg/mL),线性范围为0.25～12.0μg/mL,相关系数R=0.9993,检出限为0.16μg/mL。该方法可直接用于药物中硫普罗宁含量的测定及其药代动力学行为研究。
A new method of spectrophotometric determination of tiopronin was established. The results show that in the presence of SCN- and KNO3, Cu (I) reacted with SCN- to form CuSCN precipitate by controlling the solution pH4.0 and Cu (II) reduced by Tiopronin. The precipitate can float on the surface of water phase. By measuring the amount of Cu (II) remaining in solution, the content of tiopronin can be determined. There is a good linear relationship between absorbance and tiopronin concentration. The linear equation: A = 4.898-0.3616ρ (μg / mL), linear range of 0.25 ~ 12.0μg / mL, correlation coefficient R = 0.9993, the detection limit of 0.16μg / mL. The method can be directly used for the determination of the content of tiopronin in medicine and its pharmacokinetic behavior.