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选取北京城区及近郊４２例健康吸烟者和与之年龄、生活方式相配对的健康非吸烟者作为研究对象，分离其外周血单核细胞（ＰＢＭＣＳ），在３７℃、５％ＣＯ２条件下经植物血凝素（ＰＨＡ）刺激活化。采用免疫化学技术检测活化４８小时后的白介素２（ＩＬ－２）分泌细胞百分数、活化７２小时后的膜白介素２受体（ｍＩＬ－２Ｒ）阳性细胞百分数和血清中可溶性白介素２受体（ｓＩＬ－２Ｒ）水平。结果显示：在体外条件下，吸烟组ｍＩＬ－２Ｒ阳性细胞百分数显著高于非吸烟组（Ｐ＜０．０５）；血清中ｓＩＬ－２Ｒ水平吸烟组显著高于非吸烟组（Ｐ＜０．０５）；吸烟组ＩＬ－２分泌细胞百分数有高于非吸烟组的趋势（Ｐ＝０．０８）。指标的变化与吸烟的状况也有一定的关系。提示吸烟引起吸烟者体内作为细胞因子网络重要环节之一的ＩＬ－２及其受体系统发生改变
A total of 42 healthy smokers and healthy non-smokers matched with their age and lifestyle were selected as experimental subjects. Peripheral blood mononuclear cells (PBMCS) were isolated and cultured under the conditions of 37 ℃ and 5% CO2. Hemagglutinin (PHA) stimulates activation. The percentages of interleukin 2 (IL-2) secreting cells after 48 hours of activation were measured by immunochemical techniques. The percentage of membrane-bound interleukin 2 receptor (mIL-2R) -positive cells and soluble interleukin 2 receptor (sIL- 2R) level. The results showed that the percentage of mIL-2R positive cells in smoking group was significantly higher than that in non-smoking group (P <0.05), while the level of serum sIL-2R in smoking group was significantly higher than that in non-smoking group ). The percentage of IL-2-secreting cells in smoking group was higher than that in non-smoking group (P = 0.08). Changes in indicators and the status of smoking also have a certain relationship. Suggesting that smokers cause smokers as a cytokine network an important part of IL-2 and its receptor system changes