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Methacrylic acid first was neutralized with an aqueous solution of NaOH to pH=6.0～7.0, vinylene carbonate(VCA) was added to the solution, then monomers were copolymerized in paraffin oil by means of reverse-phase suspensionpolymerization and hydrophilic copolymeric supports were prepared. The properties of the supports were determined usingtrypsin and results show that the amount of enzymes coupled to the supports and the specific activity of immobilized trypsinare related to the content of VCA structure units, reaction time and concentration of enzyme solution, etc.
Methacrylic acid first was neutralized with an aqueous solution of NaOH to pH = 6.0-7.0, vinylene carbonate (VCA) was added to the solution, then monomers were copolymerized in paraffin oil by means of reverse-phase suspension polymerization and hydrophilic copolymeric support were prepared. The properties of the supports were determined bytrypsin and results show that the amount of enzymes coupled to the supports and the specific activity of immobilized trypsinare related to the content of VCA structure units, reaction time and concentration of enzyme solution, etc.