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1985年6—10月间,在福建省绍安县的甘薯、南京农业大学校园内盆栽的仙人掌和南京市农科所大田的芝麻上采集到甘薯(Ipomea batatas)、仙人掌(Opuntia dillenii)和芝麻(Sesamum orientale)三个丛枝病样本。分别剪取甘薯、芝麻病叶的叶柄和主脉,仙人掌的幼嫩枝条,切成1×2毫米大小的组织块。用戊二醛和锇酸双固定,经酒精梯度脱水后转换环氧丙烷三次。然后用1:3,1:1环氧丙烷:Epon812的包埋剂各浸2小时,纯Epon 812
From June to October 1985, Ipomea batatas, Opuntia dillenii and sesame seeds were collected from sweet potatoes in Shaoan County of Fujian Province, potted cactus in the campus of Nanjing Agricultural University, and sesame seeds in the field of Nanjing Agricultural Science Institute. Sesamum orientale) Three birch disease samples. Cut the sweet potato, sesame leaf petiole and main vein, cactus young shoots, cut into 1 × 2 mm size of the tissue block. Double fixation with glutaraldehyde and osmium tetroxide, dehydration of alcohol gradient conversion of propylene oxide three times. Then immersed for 2 hours with 1: 3, 1: 1 propylene oxide: Epon812 embedding agent, pure Epon 812