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选用绵羊红细胞 (SRBC)和鸡红细胞 (CRBC)作为靶细胞 ,用微量比色法检测人外周血单个核细胞 (PBMC)的抗体依赖细胞介导的细胞毒 (ADCC)活性。结果测得靶细胞对绵羊红细胞细胞毒指数为 9 0 3± 6 74 % ,对鸡红细胞的指数为 31 77± 16 2 7%。经t检验 ,有显著性差异 (t =4 75,P <0 0 1)。在 10例配对比较中 ,经相关性检验 ,对两靶细胞的指数具有显著相关性 (r =0 7152 ,P <0 0 5) ;经t检验 ,对两靶细胞的指数具有极显著差异 (t=4 33,P <0 0 1)。相同条件下 ,对CRBC的指数比SRBC的指数高。故认为 ,用CRBC作为靶细胞检测人PBMC的ADCC活性较好。
Sheep red blood cells (SRBC) and chicken erythrocytes (CRBC) were selected as target cells. Antibody dependent cell-mediated cytotoxicity (ADCC) activity of human peripheral blood mononuclear cells (PBMCs) Results The cytotoxicity index of target cells to sheep erythrocytes was 9 0 3 ± 6 74% and that of chicken erythrocytes was 31 77 ± 16 2 7%. After t test, there was a significant difference (t = 4 75, P <0.01). In the 10 paired comparisons, the correlation between the two target cells showed significant correlation (r = 0 7152, P <0 05) .Through the t-test, the indexes of the two target cells were significantly different (r = t = 4 33, P <0 0 1). Under the same conditions, the index of CRBC is higher than the index of SRBC. Therefore, using CRBC as a target cell assay ADCC activity of human PBMC better.