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切除人鼠肝左叶与中叶(68%),电镜观察术后3～120小时的肝细胞。术后20小时内肝细胞超微结构发生进行性衰退,脂滴递增,细胞器退化,而溶酶体和自噬体显著增多,胞质内的退化结构和自噬体从细胞血窦面脱落入血窦内,被巨噬细胞吞噬,此乃肝细胞在DNA复制和分裂高峰前迅速进行结构更新的表现。术后20～48小时,肝细胞处于复制和增殖高峰,也是肝细胞结构逐渐恢复的时期,脂滴减少,游离核糖体先增多,表明此时细胞以合成结构蛋白为主;继而RER等增多。术后48小时以后,肝细胞结构基本恢复正常,RER和高尔基复合体发达,提示细胞功能基本复原。肝大部切除后肝细胞超微结构变化迅烈,恢复也甚快,讨论了这些变化在肝再生中的意义。
The left lobe and middle lobe of the mice were excised (68%), and the hepatocytes were observed after 3 ~ 120 hours by electron microscopy. Within 20 hours after operation, the ultrastructures of hepatocytes developed progressive decay, lipid droplets increased, and organelles were degraded, whereas lysosomes and autophagosomes increased significantly. The degenerated structures in the cytoplasm and autophagosomes shed into the sinusoids Blood sinus, phagocytosed by macrophages, this is the rapid regeneration of the liver cells in the DNA replication and mitotic peak performance. Twenty to forty-eight hours after operation, the hepatocytes were at the peak of replication and proliferation, which was also the phase of gradual recovery of hepatocytes. The lipid droplets decreased and the free ribosome first increased, indicating that the cells were mainly composed of synthetic structural proteins at this time. After 48 hours, the structure of liver cells returned to normal, and the developed RER and Golgi complex suggested the basic recovery of cell function. After subtotal hepatectomy, the ultrastructure of hepatocytes changed rapidly and recovered quickly. The significance of these changes in liver regeneration was discussed.