以安徽地方品种“合肥黄心乌”的下胚轴、子叶、茎尖组织为外植体，在无菌条件下接种在不同植物激素的ＭＳ培养基上。在适宜的温度和光照培养条件下，获得大量的下胚轴组织形成的愈伤组织，其出愈率为７５．５％，愈伤组织转入ＭＳ＋８ｍｇ／Ｌ６ＢＡ的培养基上，分化出不定芽，但分化率很低，仅为０．５％。茎尖组织在适宜的培养基上，直接产生芽簇。这些不定芽和芽簇经切割继代培养，形成正常植株。在乌菜组织培养快速繁殖的基础上，进行耐热诱导和定向筛选。经不同温度和不同时间的处理，有１０．６％的小苗再生植株，这些植株移入大田后，在延后栽培的高温下能正常生长，但影响结实率，仅有３０％左右的植株正常结实。再生植株后代的耐热稳定性及品质在进一步试验之中。 Hypocotyl, cotyledon and shoot tip tissue of Anhui local variety Hefei huangxinwu were used as explants and inoculated on MS medium with different plant hormones under aseptic conditions. Under appropriate temperature and light culture conditions, a large number of hypocotyl tissue callus was obtained, the callus was 75.5%, callus transferred to MS + 8mg / L6BA medium, adventitious buds were differentiated , But the rate of differentiation is very low, only 0.5%. Tip tissue in the appropriate medium, directly produce bud clusters. These adventitious buds and bud clusters were subcultured to form normal plants. On the basis of rapid propagation of tissue culture in the Chinese cabbage, heat-tolerance induction and directional screening were carried out. After being treated at different temperatures and different times, 10.6% seedlings were regenerated. When these plants were transplanted into the field, they could grow normally under the high temperature of delayed cultivation but affected the seed setting rate. Only about 30% . The thermal stability and quality of the regenerated plant progeny are further tested.