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[目的]研究南极菌B-3对黄瓜的诱导抗病作用。[方法]以黄瓜幼苗为材料,研究南极菌B-3胞外寡聚糖的分子量、单糖组成,及对黄瓜叶中防御酶的诱导作用与黄瓜白粉病的诱导抗病性。[结果]B-3胞外寡聚糖经高效凝胶渗透色谱测定其分子量为2112Da,气相色谱分析由甘露糖和葡萄糖2种单糖组成;0.3%、0.5%和0.8%的B-3胞外多糖分别对黄瓜幼苗喷施不同时间后,黄瓜体内的几丁质酶,β-1,3-葡聚糖酶,苯丙氨酸解氨酶(PAL),过氧化物酶(POD)的活性均较对照有不同程度的提高,且与0.5%的壳聚糖具有相似的诱导效果;同时,0.5%的B-3多糖能够显著地降低黄瓜白粉病的病情指数,防效达24.49%。[结论]B-3寡聚糖有望开发为新型抗病诱导子。
[Objective] The research aimed to study the induced resistance of cucumber B-3 to cucumber. [Method] With cucumber seedlings as the materials, the molecular weight, monosaccharide composition and the induction of defensive enzymes in cucumber leaves and induced disease resistance of cucumber powdery mildew were studied. [Result] The molecular weight of B-3 extracellular oligosaccharides was 2112Da by high performance gel permeation chromatography. Gas chromatographic analysis consisted of two kinds of monosaccharides, mannose and glucose. B-3 cells with 0.3%, 0.5% and 0.8% Exopolysaccharide, respectively, after spraying cucumber seedlings at different times, the contents of chitinase, β-1,3-glucanase, phenylalanine ammonia lyase (PAL), peroxidase 0.5% B-3 polysaccharide can significantly reduce the cucumber powdery mildew disease index, the control effect of up to 24.49%. [Conclusion] B-3 oligosaccharides are expected to be developed as new disease-inducing elicitors.