为探讨急性丙型肝炎的临床病程和预后,作者对49例非甲非乙型肝炎病人进行了HCV标志变化的观察。49例病人均由肝组织活检诊断为急性肝炎,其中29例为输血后肝炎,20例为散发性肝炎。自发病起1、3、6、12个月时各采集一次血清标本,低温(-80.0℃)保存备查。抗-C100-3、抗-Core、第二代抗-HCV均用ELISA方法检测,抗-HCV阳性后再作定量测定,HCV RNA用套式PCR法检查。 To investigate the clinical course and prognosis of acute hepatitis C, the authors observed the change of HCV markers in 49 non-A, non-B hepatitis patients. Forty-nine patients were diagnosed as acute hepatitis by biopsy of liver tissue, of which 29 were post-transfusion hepatitis and 20 were sporadic hepatitis. Serum specimens were harvested at 1, 3, 6 and 12 months after onset, and stored at low temperature (-80.0 ℃) for future reference. Anti-C100-3, anti-Core, second-generation anti-HCV were detected by ELISA, anti-HCV positive for quantitative determination, HCV RNA nested PCR method.