目的研究丹参酮ⅡA(TanshinoneⅡA)药物血清是否具有抑制肝星状细胞(HSCs)胞浆游离钙([Ca2+]i)增高的作用。方法健康SD大鼠32只,按随机数字表随机分为4组:肝纤维化模型丹参酮ⅡA组(8只)给予用精制橄榄油配制的40%CCl4溶液,9周后腹腔注射丹参酮ⅡA(15mg/kg)15d;肝纤维化模型对照组(8只)给予用精制橄榄油配制的40%CCl4溶液,9周后灌服生理盐水15d;正常大鼠丹参酮ⅡA组(8只)仅给予等量精制橄榄油9周,然后腹腔注射丹参酮ⅡA(15mg/kg)15d;正常对照组(8只)仅给予等量精制橄榄油,9周后灌服生理盐水15d。结束后,经下腔静脉取血并分离血清。采用盲法,用上述10%血清培养HSCs24h并负载好Fluo23PAM后使用激光扫描共聚焦显微镜(LSCM)检测HSCs[Ca2+]i。结果①经正常大鼠丹参酮ⅡA组及肝纤维化模型丹参酮ⅡA组的血清预处理的HSCs,其[Ca2+]i荧光强度相对值均明显低于肝纤维化模型对照组(P<0.05);且二者与正常对照组相比也均差异有统计学意义(P<0.05)。②经正常大鼠丹参酮ⅡA组及肝纤维化模型丹参酮ⅡA组血清预处理HSCs后加入AngⅡ,HSCs[Ca2+]i荧光强度变化百分数显著低于肝纤维化模型对照组(P<0.01)。结论丹参酮ⅡA能抑制肝星状细胞活化[Ca2+]i的升高,可能是其发挥抗肝纤维化作用的重要途径之一。 Objective To investigate whether TanshinoneⅡA serum can inhibit the increase of cytosolic free calcium ([Ca2 +] i) in hepatic stellate cells (HSCs). Methods Thirty-two healthy SD rats were randomly divided into 4 groups according to a random number table: Tanshinone ⅡA group (n = 8) was given 40% CCl4 solution with refined olive oil. After 9 weeks, tanshinone ⅡA / kg) for 15 days. The liver fibrosis model control group (n = 8) was given 40% CCl4 solution with refined olive oil. After 9 weeks, normal saline was given for 15 days. Tanshinone IIA group (n = 8) Olive oil was purified for 9 weeks and then intraperitoneally injected with tanshinone IIA (15 mg / kg) for 15 days. Normal control group (8) received only equal amount of refined olive oil and saline for 15 days after 9 weeks. After the end, blood is drawn through the inferior vena cava and serum is separated. The HSCs [Ca2 +] i were detected by laser scanning confocal microscopy (LSCM) after blinded with HSCs for 24 h with 10% serum and loaded with Fluo23PAM. Results ① The relative fluorescence intensity of [Ca2 +] i in serum pretreated HSCs from tanshinone ⅡA group and tanshinone ⅡA group was significantly lower than that of liver fibrosis model group (P <0.05); and The difference between the two groups was also statistically significant (P <0.05). ② Serum pretreatment of HSCs with tanshinone ⅡA group and tanshinone ⅡA group of rats with hepatic fibrosis induced AngⅡ, the percentage of change of [Ca2 +] i fluorescence intensity of HSCs was significantly lower than that of liver fibrosis model control group (P <0.01). Conclusion Tanshinone ⅡA can inhibit the activation of [Ca2 +] i in hepatic stellate cells, which may be one of the important ways to exert anti-hepatic fibrosis.