乙型肝炎病毒(HBV)感染能导致急、慢性肝病,其中全球慢性乙型肝炎感染者近4亿。每年由于HBV慢性感染引起的肝硬化,肝细胞癌死亡患者超过47万人。而由于HBV病毒复制过程缺乏校对机制,在慢性感染的过程中发生基因突变的概率很高,相关研究最多的HBV突变株主要有：发生在翻译水平的前C区突变(G1896A)；发生在核心启动子(BCP)区,即A1762T和G1764A的联合突变；发生在HBV DNA Hepatitis B virus (HBV) infection can cause acute and chronic liver disease, of which nearly 400000000 global chronic hepatitis B infection. Every year due to cirrhosis of the liver caused by chronic HBV infection, more than 470,000 people die of hepatocellular carcinoma. However, due to the lack of proofreading mechanism in HBV replication process, the probability of gene mutation in chronic infection is high. The most frequently studied HBV mutants include: Pre-C mutation (G1896A) at translation level; Promoter (BCP) region, a combined mutation of A1762T and G1764A; occurs in HBV DNA