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目的建立一种接近人类特征的严重创伤多器官功能不全综合征(MODS)动物模型。方法 60只SD大鼠随机分为创伤MODS模型组及正常对照组,雌雄各半,每组30只。创伤MODS模型组大鼠30％体表面积III度烫伤6 h后,腹腔注射大剂量内毒素(LPS)。正常对照组除不进行烫伤和腹腔注射内毒素外,其他处理方式与创伤MODS模型组相同。观察创伤MODS模型组及正常对照组伤后各时相点死亡例数和7 d内死亡率、以及伤后各时相点MODS相关指标[血丙氨酸氨基转移酶(ALT),肌酸激酶(CK),尿素氮(BUN),空腹血糖(FGLU)等]和促炎症细胞因子(血TNFα,NO和IL-6)水平动态变化。结果创伤MODS模型组大鼠死亡主要发生在烫伤1 d后,7 d内死亡率为53．33％,烫伤1 d后死亡占总死亡例数的87．5％,正常对照组无死亡。创伤 MODS模型组伤后各时相点血ALT,CK,BUN,FGLU等MODS相关指标以及血TNFα,NO和 IL-6水平均明显高于正常对照组(P<0．0001)。结论严重创伤后腹腔注射大剂量内毒素,可成功复制创伤MODS动物模型。
Objective To establish a human model of severe traumatic multiple organ dysfunction syndrome (MODS). Methods Sixty SD rats were randomly divided into traumatic MODS model group and normal control group, with 30 male and one female. Rats in trauma MODS model group were injected intraperitoneally with high dose of lipopolysaccharide (LPS) after intraperitoneal injection of 30% body surface area for 3 years. The normal control group except burn and intraperitoneal injection of endotoxin, other treatment and traumatic MODS model group the same. The number of deaths at each time point and the rate of death within 7 days after trauma MODS model group and normal control group were observed, and the indexes of MODS (ALT, creatine kinase (CK), blood urea nitrogen (BUN), fasting blood glucose (FGLU), etc.) and the levels of proinflammatory cytokines (blood TNFα, NO and IL-6) Results The death of MODS model rats occurred mainly in the first day after scald, the death rate was 53.33% within 7 days and the death rate was 87.5% of the total number of deaths after 1 day of scald injury. There was no death in the normal control group. The levels of ALT, CK, BUN, FGLU and other MODS and the levels of TNFα, NO and IL-6 in trauma MODS model group were significantly higher than those in normal control group (P <0.0001). Conclusion Severe traumatic intraperitoneal injection of large doses of endotoxin, can successfully replicate traumatic MODS animal model.