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目的探讨二甲基甲酰胺(dimethylformamide,DMF)对正常心肌细胞(H9C2)的细胞毒性作用。方法采用CCK-8法检测不同浓度梯度(0 mmol/L,50 mmol/L,100 mmol/L,150 mmol/L,200 mmol/L,250 mmol/L,300 mmol/L)DMF作用心肌细胞24 h、48 h、72 h后的细胞毒性,并用Annexin V-FITC/PI流式细胞术检测不同时间(24 h、48 h、72 h)和浓度(24 h的染毒剂量为0 mmol/L,100 mmol/L,140 mmol/L,180 mmol/L,220 mmol/L,250 mmol/L;48 h的染毒剂量为0 mmol/L,100 mmol/L,140 mmol/L,180 mmol/L,200 mmol/L,220 mmol/L;72 h的染毒剂量为0 mmol/L,100 mmol/L,125 mmol/L,140 mmol/L,180 mmol/L,200 mmol/L)的细胞凋亡率。结果不同浓度DMF作用于心肌细胞24 h,48 h和72 h后产生了明显的细胞毒性。同一染毒时间组内,不同剂量组的细胞存活率差异有统计学意义(均有P<0.05);同一染毒剂量组内,各不同染毒时间组的细胞存活率差异均有统计学意义(均有P<0.05)。用CCK-8法检测DMF作用于心肌细胞的24 h IC50是250 mmol/L,48 h IC50是220 mmol/L,72 h IC50是200 mmol/L。细胞凋亡趋势与其对细胞的毒性作用结果一致。结论与阴性组比较,DMF对心肌细胞产生了细胞损伤,具有细胞毒性。
Objective To investigate the cytotoxic effect of dimethylformamide (DMF) on normal cardiomyocytes (H9C2). Methods CCK-8 was used to detect the effect of different concentrations of DMF (0 mmol / L, 50 mmol / L, 100 mmol / L, 150 mmol / L, 200 mmol / L, 250 mmol / L, 300 mmol / L) (24 h, 48 h, 72 h) and 24 h (24 h, 48 h, 72 h) were measured by Annexin V-FITC / PI flow cytometry. L, 100 mmol / L, 140 mmol / L, 180 mmol / L, 220 mmol / L, 250 mmol / L and 48 h were 0 mmol / L, 100 mmol / L, 140 mmol / L, 180 100 mmol / L, 125 mmol / L, 140 mmol / L, 180 mmol / L and 200 mmol / L for 72 h ) Rate of apoptosis. Results After 24 h, 48 h and 72 h, different concentrations of DMF produced obvious cytotoxicity. Within the same exposure time group, cell survival rates of different dose groups were statistically significant (both P <0.05); within the same exposure dose group, the cell survival rates of different exposure time groups were statistically significant (All P <0.05). The 24 h IC50 of DMF on cardiomyocytes measured by CCK-8 method was 250 mmol / L, IC 50 was 220 mmol / L at 48 h, and IC 50 was 200 mmol / L at 72 h. The trend of apoptosis is consistent with the cytotoxic effect on cells. Conclusion Compared with the negative group, DMF has cytotoxicity on cardiomyocytes.