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目的:检测精神分裂症模型小鼠脑内c-Fos与尼克酰胺腺啶呤二核苷酸磷酸黄递酶(NADPH-diapho-rase,NADPH-d)的共存,探讨一氧化氮(nitricoxide,NO)与精神分裂症的关系,为深入研究精神分裂症的发病机制提供形态学和神经化学依据。方法:雄性昆明小鼠,随机分为生理盐水组和苯环己哌啶(phencyclidine,PCP)组,分别用自主活动观察、Sams-Dodd的刻板行为评分标准对模型小鼠的行为改变进行检测,同时用NADPH-d组织化学和c-Fos免疫组织化学双染法检测各组小鼠脑内c-Fos/NADPH-d阳性神经元的分布。结果:(1)PCP组小鼠出现明显的自发活动的增加(P<0.01)及显著的刻板行为(P<0.01);(2)PCP组小鼠脑内c-Fos与NADPH-d阳性神经元单纯表达的数量明显高于对照组,并且在伏隔核、屏状核及被盖背外侧核出现较多c-Fos/NADPH-d双标阳性神经元,生理盐水组小鼠脑内未见c-Fos/NADPH-d双标阳性神经元。结论:NO可能在谷氨酸NMDA受体功能异常引起的精神分裂症的病理过程发挥一定作用。
Objective: To investigate the coexistence of c-Fos and NADPH-diacetate (NADPH-d) in the brain of schizophrenic model mice and to explore the relationship between nitric oxide (NO) ) And schizophrenia, provide a morphological and neurochemical basis for further study of the pathogenesis of schizophrenia. Methods: Male Kunming mice were randomly divided into normal saline group and phencyclidine (PCP) group. The behavioral changes of the model mice were detected by autonomic activity and Sams-Dodd’s stereotypical score scale. At the same time, the distribution of c-Fos / NADPH-d positive neurons in each group was detected by NADPH-d histochemistry and c-Fos immunohistochemical double staining. (2) PC-Fos and NADPH-d positive neurons in the PCP group were significantly higher than those in the PCP group (P <0.01) Compared with the control group, the number of simple expression of the c-Fos / NADPH-d double positive neurons in the nucleus accumbens, the nucleus accumbens and the dorsomedial nucleus was significantly higher than that in the control group See c-Fos / NADPH-d double positive neurons. CONCLUSIONS: NO may play a role in the pathological process of schizophrenia caused by dysfunction of glutamate NMDA receptor.