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目的了解宁波地区食品中携带或污染的致病菌,为控制食源性疾病提供依据。方法致病菌检测采用直接分离与增菌分离相结合的方法;细菌鉴定采用生化筛检和API等方法;血清分型采用诊断血清凝集法;药敏试验采用K-B法;采用PCR检测耐药基因。结果从6 812份食品标本中检出致病菌7类12种,共2 331株,检出率为34.22%,以副溶血性弧菌检出率最高,与其他病原菌检出率比较差异有统计学意义(P<0.005)。主要流行株副溶血性弧菌分型发现10个血清群,O∶6群和O∶5群为优势群。检出的致病菌对大多数抗生素敏感,其中3株气单胞菌为带aacc耐药基因的多重耐药菌。结论宁波地区食品中致病菌构成复杂,食品污染是引起食源性疾病的主要原因,其中副溶血性弧菌是最主要的流行菌株;检出的致病菌对多种抗生素敏感,存在aacc耐药菌应引起关注,控制的关键是采取合理用药,加强对耐药菌株的监测,以减少耐药菌的传播和扩散。
Objective To understand the pathogenic bacteria carried in or contaminated by food in Ningbo and provide the basis for controlling foodborne diseases. Methods The pathogenic bacteria were detected by the method of direct separation combined with the enrichment of bacteria. The bacteria were identified by biochemical screening and API, the serum typing was by serum agglutination, the susceptibility test by KB method, . Results Among 6 812 food samples, 12 kinds of 7 kinds of pathogens were detected, of which 2 331 strains were detected, the detection rate was 34.22%. The detection rate of Vibrio parahaemolyticus was the highest, and the detection rate of other pathogenic bacteria was Statistical significance (P <0.005). Vibrio parahaemolyticus strains of major epidemic strains found 10 serogroups, O: 6 group and O: 5 group as the dominant group. The pathogens detected were sensitive to most antibiotics, of which 3 Aeromonas were multi-drug resistant strains with aacc resistance gene. Conclusions The pathogenic bacteria in food in Ningbo are complex and food contamination is the main cause of foodborne diseases. Vibrio parahaemolyticus is the most important epidemic strain. The pathogenic bacteria detected are sensitive to many antibiotics. There are aacc Drug-resistant bacteria should be concerned about the key control is to take reasonable medication to strengthen the monitoring of drug-resistant strains to reduce the spread and spread of resistant bacteria.