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应用免疫组织化学方法(ABC法)观察了阿片μ受体(mu-opioidreceptor,MOR)在猫脊髓和脊神经节内的分布。在脊髓内,MOR样免疫反应产物主要分布于神经毯(neuropil)内,偶尔可见阳性胞体。致密的MOR样免疫反应产物主要分布于背角的Ⅰ、ⅠⅠ层。在胸腰髓和骶髓的中间带外侧核、中央管周围灰质、骶髓节段的后连合核和Onuf核内发现有中等密度的MOR样免疫反应产物。背索外侧部的Lissauer束内的纤维亦呈现中等密度的免疫阳性染色。此外,脊神经节内分布大量MOR样免疫反应细胞。切断后根后,发现术侧背角浅层内MOR样免疫反应产物密度有明显降低;而半横断脊髓后,切断平面上下脊髓背角浅层内MOR样免疫反应产物密度无明显改变。上述结果表明,阿片μ受体在猫脊髓内主要分布于背角浅层,终止于背角浅层的外周传入纤维中含有MOR。
Immunohistochemistry (ABC method) was used to observe the distribution of mu opioid receptor (MOR) in the spinal cord and spinal ganglia of cats. Within the spinal cord, the MOR-like immunoreactive products are predominantly localized in the neuropil, with occasional positive soma. The dense MOR-like immunoreactive products mainly distributed in the Ⅰ, Ⅰ Ⅰ layer of the dorsal horn. Median density MOR-like immunoreactive products were found in the medullar lateral nucleus of the thoracolumbar and sacral medulla, the periventricular gray matter, the posterior commissural nucleus of the sacral canal segments, and the Onuf nucleus. Fibers within the Lissauer bundle outside the dorsal cord also showed medium density immunopositive staining. In addition, a large number of MOR-like immunoreactive cells are distributed in the spinal ganglia. After cutting the posterior root, the density of MOR-like immunoreactive products in the superficial layers of the dorsal horn at the operative side was significantly reduced. However, the density of MOR-like immunoreactive products in the superficial layers of the spinal dorsal horn at the cut-off plane had no significant changes after the transection of the spinal cord. The above results indicate that opioid mu receptors are mainly located in the superficial layer of dorsal horn in the spinal cord of the cat, and the peripheral afferent fibers terminating in the superficial dorsal horn contain MOR.