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通过柱色谱法从马铃薯叶的提取物中分离提纯了茄尼醇,并以RP-HPLC法测定了茄尼醇的质量分数。采用硅胶对茄尼醇进行吸附纯化,以茄尼醇的实测收率、质量分数为考察指标,通过对硅胶颗粒、洗脱剂流速、洗脱剂组分配比进行考察,确定了茄尼醇的柱色谱优化条件。结果表明,以200~300目硅胶为填料,流动相流速为2.0 mL/m in,洗脱剂以石油醚、V(石油醚)∶V(乙酸乙酯)=20∶1、15∶1、9∶1进行梯度洗脱,茄尼醇的质量分数达到85.37%,重结晶后达到97.47%。
Solanesol was isolated and purified from the leaves of potato by column chromatography, and the mass fraction of solanesol was determined by RP-HPLC. The solanesol was adsorbed and purified by silica gel. The measured yield and mass fraction of solanesol were investigated. Through the investigation of the silica gel particles, the eluent flow rate and the eluent composition ratio, Column chromatography conditions. The results showed that the flow rate of mobile phase was 2.0 mL / min with silica gel of 200-300 mesh as the eluent, and petroleum ether, V (petroleum ether): V (ethyl acetate) = 20:1, 9: 1 gradient elution, solanesol mass fraction reached 85.37%, after recrystallization reached 97.47%.