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目的研究内吗啡肽(EM)对“泻剂结肠”大鼠结肠动力的影响,以探讨慢传输性便秘(STC)的发病机制。方法以“泻剂结肠”大鼠为模型,用电刺激离体肌条收缩反应试验方法观察EM对离体肌条的影响。结果与对照组相比,EM1和EM2明显抑制电刺激“泻剂结肠”大鼠离体肌条收缩反应,收缩波振幅降低,对远端结肠抑制尤其明显;EM1的抑制作用强于EM2,这种抑制作用与浓度相关,不同浓度的Naloxone(u阿片受体拮抗剂)明显加强EM作用后电刺激“泻剂结肠”大鼠离体肌条的收缩反应,收缩波振幅增加。结论EM1和EM2参与“泻剂结肠”动力的调控,可能是STC发病的一个重要因素。
Objective To study the effect of endomorphin (EM) on colonic motility of “cathartic colon” rats and to explore the pathogenesis of slow transit constipation (STC). Methods “Laxative colon” rat model was used to observe the effect of EM on isolated muscle strips by electrical stimulation of contractions of muscle strips in vitro. Results Compared with the control group, EM1 and EM2 significantly inhibited the contractile response in vitro and the contractile amplitude of contractile wave in electrical stimulation of “laxative colon”, especially for distal colon; the inhibitory effect of EM1 was stronger than that of EM2 The inhibitory effect was related to the concentration. Different concentrations of Naloxone (u opioid receptor antagonist) significantly enhanced the contractile response of isolated muscle strips of rats with laxative colon after electrical stimulation with EM, and the amplitude of contraction wave increased. Conclusion EM1 and EM2 are involved in the regulation of motility of “laxative colon” and may be an important factor in the pathogenesis of STC.