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通过降低反应物中L-半胱氨酸(L-Cys)的比例,在水相快速合成了近红外CdTe量子点,使之对巯基化合物产生荧光响应.并以此构建了一种基于表面配体缺失的CdTe近红外荧光量子点的巯基探针,为生物样品中的硫醇检测提供简便经济、高灵敏度和高选择性的新方法.在其它多种氨基酸和生物液体中主要离子、分子共存的情况下,我们所制备的近红外量子点对L-Cys、同型半胱氨酸(Hcy)和谷胱甘肽(GSH)的荧光检测中显示了良好的选择性和灵敏度.在血清和细胞提取液中,加标5.0μmol?L-1硫醇的回收率均在90%~109%范围内.该方法对L-Cys,Hcy和GSH的检出限(3s)分别为43,46和63 nmol?L-1.
By reducing the ratio of L-Cys in the reactants, near-infrared CdTe QDs were rapidly synthesized in the aqueous phase to generate a fluorescence response to the thiol compounds, Lack of CdTe near infrared fluorescent QDs thiol probe for the detection of thiols in biological samples provides a simple and economical, high sensitivity and high selectivity of the new method.In many other amino acids and biological fluids, the main ions and molecules coexist , The prepared NIR quantum dots showed good selectivity and sensitivity in the fluorescence detection of L-Cys, homocysteine (Hcy) and glutathione (GSH) .In the serum and cells The recoveries of spiked 5.0 μmol · L-1 thiols in the extract ranged from 90% to 109% .The detection limits (3s) for L-Cys, Hcy and GSH were 43, 46 and 63 nmol? L-1.