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目的 :为了探讨异种器官移植中T细胞参与的免疫排斥机理 ,从健康人外周血中试行建立识别猪淋巴细胞抗原特异性的T细胞系方法研究。方法 :通过照光致弱的猪外周血单个核细胞反复刺激人的T淋巴细胞 ,3H TdR掺入法筛选特异性增殖的T细胞。结果 :获得 2株为TCRαβ+CD3+CD8+细胞 ,另 1株为TCRαβ+CD3+CD4+细胞。结论 :健康人外周血中有识别异种抗原特异性的T细胞系 ,但出现频率较低
OBJECTIVE: To investigate the immune rejection mechanism involved in T cell transplantation in xenotransplantation and to establish a method to identify the T lymphocyte-specific T cell line from healthy human peripheral blood. Methods: Human T lymphocytes were stimulated repeatedly by attenuated porcine peripheral blood mononuclear cells, and 3H - TdR incorporation method was used to screen specific proliferating T cells. Results: Two strains of TCRαβ + CD3 + CD8 + cells were obtained and the other one was TCRαβ + CD3 + CD4 + cells. Conclusion: There are some T cell lines in the peripheral blood of healthy people which recognize the specific antigen of different species, but their frequencies are lower