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目的运用组织芯片及常规技术检测活化的细胞毒性细胞在各型淋巴瘤中的表达分布情况,为临床治疗和判断预后提供依据。方法运用免疫组织化学方法检测以60例淋巴瘤标本制成的淋巴瘤组织芯片中穿孔素、颗粒酶B的表达和分布情况;同时选择10例鼻NK/T细胞淋巴瘤常规标本与组织芯片进行比较研究,10例淋巴组织反应性增生作为对照。结果在60例淋巴瘤制成的组织芯片中,发生在淋巴结内者48例,淋巴结外者12例。B细胞淋巴瘤42例,T细胞淋巴瘤16例,2例霍奇金淋巴瘤。42例B细胞淋巴瘤的瘤细胞均不表达穿孔素和颗粒酶B。10例外周T细胞淋巴瘤中8例表达穿孔素和9例表达颗粒酶B,阳性表达细胞均为非肿瘤细胞。芯片中的2例和常规10例鼻NK/T细胞淋巴瘤中均见穿孔素和颗粒酶B过度表达。T、B细胞淋巴瘤与NK/T细胞淋巴瘤比较有显著性差异(P<0.01)。结论穿孔素和颗粒酶B是鉴定活化的细胞毒性细胞的免疫标志物,可作为NK/T细胞淋巴瘤的诊断性标志物;其在T细胞淋巴瘤中的表达反映了机体存在抗肿瘤的免疫反应机制。组织芯片技术具有高信息量、简捷、高效、实验误差小、重复性好等优点,可作为研究淋巴瘤的有用工具。
Objective To detect the expression and distribution of activated cytotoxic cells in various types of lymphomas by tissue microarray and routine techniques to provide evidence for clinical treatment and prognosis. Methods Immunohistochemistry was used to detect the expression and distribution of perforin and granzyme B in lymphoma tissue samples from 60 lymphoma specimens. Ten specimens of nasal NK / T cell lymphoma were selected for tissue engineering Comparative study, 10 cases of lymphoid reactive hyperplasia as a control. Results in 60 cases made of lymphoma tissue microarray, occurred in the lymph node in 48 cases, 12 cases of lymph node outside. 42 B-cell lymphoma, 16 T-cell lymphoma and 2 Hodgkin’s lymphoma. The tumor cells of 42 B-cell lymphomas did not express perforin and granzyme B. In 10 cases of peripheral T cell lymphoma, 8 cases expressed perforin and 9 cases expressed granzyme B, the positive cells were all non-tumor cells. Perforin and granzyme B overexpression were seen in 2 of the chips and 10 of the conventional nasal NK / T cell lymphomas. T, B cell lymphoma and NK / T cell lymphoma were significantly different (P <0.01). Conclusion Perforin and granzyme B are the immunological markers for identifying activated cytotoxic cells and can be used as diagnostic markers for NK / T cell lymphoma. The expression of perforin and granzyme B in T cell lymphoma reflects the presence of anti-tumor immunity in the body Reaction mechanism. Tissue chip technology has the advantages of high information content, simple and efficient, small experimental error and good repeatability, which can be used as a useful tool to study lymphoma.