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目的探讨基质金属蛋白酶-13(MMP-13)及MMP-13 mRNA在人肝细胞肝癌(HCC)中的表达和意义。方法分别应用免疫组化和原位杂交方法检测32例HCC患者手术切除的癌组织及其癌旁组织中的MMP-13蛋白和MMP-13 mRNA,分析临床病理资料与MMP-13的关系。结果HCC组织中MMP-13和MMP-13 mRNA阳性表达率为93.75%(30/32)和90.63%(29/32),癌旁组织中的阳性表达率为71.88%(23/32)和59.38%(19/32),两组比较差异有统计学意义(P<0.01)。MMP-13在肿瘤中的表达与肿瘤组织分化程度、有无门静脉癌栓和包膜侵犯密切相关(P<0.05)。结论MMP-13及MMP-13 mRNA在HCC组织中的表达明显高于癌旁组织,且与组织分化、门脉癌栓等临床病理有关,提示MMP-13在肿瘤的生长过程中发挥一定作用,可能与HCC的侵袭和转移有关。
Objective To investigate the expression and significance of matrix metalloproteinase-13 (MMP-13) and MMP-13 mRNA in human hepatocellular carcinoma (HCC). Methods Immunohistochemistry and in situ hybridization were used to detect MMP-13 protein and MMP-13 mRNA in 32 cases of HCC patients and their adjacent tissues. The relationship between clinicopathological data and MMP-13 was analyzed. Results The positive rates of MMP-13 and MMP-13 mRNA in HCC tissues were 93.75% (30/32) and 90.63% (29/32), respectively. The positive rates of MMP-13 and MMP-13 mRNA in HCC tissues were 71.88% % (19/32), the difference between the two groups was statistically significant (P <0.01). The expression of MMP-13 in the tumor was closely related to the degree of differentiation of the tumor, presence or absence of portal vein thrombosis and invasion of the capsule (P <0.05). Conclusions The expression of MMP-13 and MMP-13 mRNA in HCC tissues is significantly higher than that in paracancerous tissues, and is related to the clinicopathological features such as tissue differentiation and portal vein tumor thrombus, suggesting that MMP-13 plays a role in tumor growth, May be related to the invasion and metastasis of HCC.