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引言体细胞杂交已成为引导人们绕过有性杂交育种的障碍,利用那些有性杂交不亲和,但又十分重要的种质资源来进行作物改良的一种手段。然而,在这一领域中,成功地应用大多局限在茄科(Collins 等1984)及近来报道的十字花科(Sundberg 和 Glimeli-us 1986),原因很多,其中包括,除茄科外,在其它科植物上缺乏从原生质体到植株再生的有效系统;在亲缘关系较远的种间具有体细胞不亲和性(Harm 1983)。柑桔
Introduction Somatic hybridization has become a tool to guide people to bypass sexual crossbreeding and make use of those unfavorable sexually cross-linked but important germplasm resources for crop improvement. However, in this area, the successful application of the crucifer (Sundberg and Glimeli-us 1986), mostly confined to the Solanaceae family (Collins et al. 1984) and more recently, has many causes, including, in addition to Solanaceae, Families lack effective systems for regeneration from protoplasts to plants; there is somatic incompatibility among distantly related species (Harm 1983). Citrus