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目的:建立测定麻杏石甘汤中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱含量的毛细管电泳法。方法:采用未涂层弹性融硅石英毛细管柱(60 cm×55μm ID,有效长度52 cm);以60 mmol/L硼砂+10%(体积分数)甲醇(pH 9.0)为运行缓冲液;分离电压12 kV;重力进样10 s(高度15 cm);检测波长210 nm;以盐酸小檗碱为内标。结果:盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱浓度分别在20.0~160.0μg/mL(r=0.9999)、7.5~60.0μg/mL(r=0.9991)、2.0~10.0μg/mL(r=0.9993)内线性关系良好,3种成分的平均加样回收率依次为98.0%、97.0%、97.8%,方法精密度(RSD)依次为2.31%、2.21%、2.00%(n=6)。结论:本方法简便、快速,结果准确可靠,可用于麻杏石甘汤的质量控制。
Objective: To establish a capillary electrophoresis method for determination of content of ephedrine hydrochloride, pseudoephedrine hydrochloride and methyl ephedrine hydrochloride in Maxingshigan Decoction. Methods: Uncoated flexible fused silica capillary column (60 cm × 55 μm ID with an effective length of 52 cm) was used as running buffer with 60 mmol / L borax and 10% methanol (pH 9.0) as the running buffer. The separation voltage 12 kV; gravity injection 10 s (height 15 cm); detection wavelength 210 nm; berberine hydrochloride as internal standard. Results: The concentrations of ephedrine hydrochloride, pseudoephedrine hydrochloride and methyl ephedrine hydrochloride were 20.0 ~ 160.0μg / mL (r = 0.9999), 7.5 ~ 60.0μg / mL (r = 0.9991) and 2.0 ~ 10.0μg / mL 0.9993). The average recoveries of three components were 98.0%, 97.0% and 97.8%, respectively. The method precision was 2.31%, 2.21% and 2.00%, respectively (n = 6). Conclusion: The method is simple, rapid, accurate and reliable, and can be used for the quality control of Maxing Shigan Decoction.