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[目的]为比较三组概率分析法和传统QTL定位法,建立高效稳定的SSR扩增技术体系,筛选出在双亲间具有多态性的引物。[方法]本研究利用水稻Ⅱ-32B和特青两个亲本对605对均匀分布于12条染色体的SSR引物的扩增情况进行了初步筛选,并研究了其扩增多态性和最优SSR-PCR反应体系[结果]结果表明:适宜水稻的SSR-PCR反应体系(10μl)为10×Buffer 2μl,Mg2+终浓度2.0 mmol/L,dNTP终浓度0.5 mmol/L,引物终浓度1.0μmol/L,DNA模板1μl,Taq DNA聚合酶0.15U。从分布于全基因组的SSR引物中筛选出142对在双亲间具有多态性的引物。[结论]为完成后续的QTL定位研究奠定良好的基础。
[Objective] The research aimed to establish a highly efficient and stable SSR amplification system for the comparison of three sets of probability analysis and traditional QTL mapping methods and screen out the primers with polymorphisms between parents. [Method] The preliminary screening of 605 pairs of SSR primers uniformly distributed on 12 chromosomes in rice Ⅱ-32B and Teqing was conducted in this study. The amplification polymorphism and SSR PCR reaction system [Results] The results showed that the optimum reaction conditions were as follows: 10 μl of 10 μl buffer, 2 μl of final concentration of Mg2 +, 0.5 mmol / L of dNTP and 1.0 μmol / L of primer 1 μl DNA template and 0.15 U Taq DNA polymerase. From the SSR primers distributed on the whole genome, 142 pairs of primers with polymorphisms between parents were screened out. [Conclusion] The study laid a good foundation for the follow-up QTL mapping.