论文部分内容阅读
采用含ＰＥＧ的稀释液稀释酶标记物，ＥＬＩＳＡ温育时间可缩短到３７℃１５分钟，检测二肝“两对半”其持异性达到１００％，ＨＢｓＡｇ最低检出量为ｌｎｇ／ｍｌ，抗－ＨＢｓ为２ｍＵ／ｍｌ，ＨＢｅＡｇ的３种参比血清（３７＃，４９＃，５６＃），抗ＨＢｅ的３种参比血清（５５＃，５７＃，６１＃），抗。ＨＢｃ的３种参比血情（２＃，３＃，４＃），部颁的最高稀释度均可测出，１５分钟法对乙肝标志物检出稀释度高于常规法，批内变异ＣＶ３．１４％～１４．４７％，批间变异ＣＶ５．６１％～９．０９％，冷（４℃），热（３７℃）气温操作结果与室温操作结果无显著性差异，稀释的酶标记物于４℃保存６个月仍保持活性。
The dilution of the enzyme-labeled substance with PEG-containing diluent can shorten the incubation time to 37 ° C for 15 minutes. Detection of Hepatic Two-and-Half Hepatitis B with 100% of its heterosexuality, the detection limit of HBsAg is 1 ng / ml, Three reference serums (37 #, 49 #, 56 #) with HBs of 2 mU / ml, HBeAg, and three reference serums (55 #, 57 #, 61 #) against HBe were tested. HBc 3 reference blood (2 #, 3 #, 4 #), ministerial highest dilution can be measured, 15-minute method of detection of hepatitis B markers diluted higher than the conventional method, intra-assay CV3 .14% ~ 14.47%, CV5.61% ~ 9.09% between batch variations, cold (4 ℃), hot (37 ℃) temperature operating results and room temperature operating results no significant difference, diluted enzyme markers Store at 4 ° C for 6 months and remain active.