目的探讨花生四烯酸和亚油酸对大鼠肝星状细胞（ＨＳＣ）增殖的影响．方法用链霉蛋白酶和胶原酶原位灌流，ｎｙｃｏｄｅｎｚ密度梯度离心分离大鼠肝星状细胞，并以ＭＴＴ比色法观察花生四烯酸和亚油酸对ＨＳＣ增殖效应．结果花生四烯酸和亚油酸可影响ＨＳＣ的增殖．花生四烯酸２５ｍｇ／Ｌ对ＨＳＣ有增殖作用（Ｐ＜００１），而５０，１００ｍｇ／Ｌ对ＨＳＣ增殖有抑制作用（Ｐ＜００１），倒置显微镜观察可见ＨＳＣ增殖非常缓慢，ＨＳＣ细胞轮廓非常明显，胞质粗糙，内有颗粒沉积，１２５，６２５ｍｇ／Ｌ对ＨＳＣ增殖无影响．亚油酸６２５，１２５，２５ｍｇ／Ｌ对ＨＳＣ增殖无明显影响（Ｐ＞００５），但随着浓度增大，５０，１００ｍｇ／Ｌ则促进ＨＳＣ增殖（Ｐ＜００５或Ｐ＜００１）．结论花生四烯酸和亚油酸在一定浓度下可促进肝星状细胞增殖，高浓度对ＨＳＣ有毒性作用，其可能通过脂质过氧化作用而与脂肪肝肝纤维化的发生有关 Objective To investigate the effects of arachidonic acid and linoleic acid on the proliferation of rat hepatic stellate cells (HSCs). Methods In situ perfusion with pronase and collagenase, isolated rat hepatic stellate cells by nycodenz density gradient centrifugation, and the effects of arachidonic acid and linoleic acid on HSC proliferation were observed by MTT assay. Results Arachidonic acid and linoleic acid can affect the proliferation of HSC. Arachidonic acid 25mg / L had a proliferative effect on HSC (P <001), while 50,100mg / L inhibited the proliferation of HSC (P <001). The inverted microscope showed that proliferation of HSC was very slow. HSC Cell outline is very clear, rough cytoplasm, there are particles deposited, 12  5,6  25mg / L HSC proliferation had no effect. Linoleic acid 6  25,12  5,25 mg / L had no significant effect on HSC proliferation (P> 0  05), but with the increase of concentration, 50 and 100 mg / L promoted the proliferation of HSC (P <005 Or P <001). Conclusions Arachidonic acid and linoleic acid can promote the proliferation of hepatic stellate cells at a certain concentration. The high concentrations of arachidonic acid and linoleic acid have a toxic effect on HSC, which may be related to the occurrence of hepatic fibrosis of fatty liver by lipid peroxidation