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利用20个微卫星标记对5个大鳞副泥鳅(Paramisgurnus dabryanus)家系的遗传结构进行分析,探究家系遗传多样性及其亲缘关系的远近,为进一步遗传选育提供技术参数。结果显示,20个微卫星标记在5个家系中共检测到等位基因数53个,平均等位基因数2.65个,各位点的等位基因数2~4个;5个家系平均观测杂合度(Ho)0.3117~0.5542,平均期望杂合度(He)0.3261~0.4371,平均多态信息含量(PIC)0.3459~0.4538,平均固定系数(Fis)-0.0472(<0),遗传分化系数(Fst)0.2703;UPGMA聚类分析显示,AB2013F-7和AB2013F-81亲缘关系最远。研究表明,5个家系间遗传分化较大,具备较大的选育空间。
Twenty microsatellite markers were used to analyze the genetic structure of five Paramisgurnus dabryanus families, to explore the genetic diversity of families and their genetic distance, and to provide technical parameters for further genetic selection. The results showed that there were 53 alleles, the average number of alleles was 2.65 and the number of alleles was 2-4 in all 5 families. The average observed heterozygosity The average expected heterozygosity (He) was 0.3261 ~ 0.4371, the average polymorphism information content (PIC) was 0.3459 ~ 0.4538, the average fixed coefficient was -0.0472 (<0) and the genetic differentiation coefficient was 0.703. UPGMA cluster analysis showed that AB2013F-7 and AB2013F-81 were the most distantly related. The research shows that the genetic differentiation among five families is larger and has more space for breeding.