目的　揭示两性分子与有序磷脂膜的作用模式。方法　分别使用磷脂膜色谱和正辛醇 水系统测定药物的膜亲和性和疏水性参数。结果　两性分子与有序磷脂膜存在吸引性极性附加作用力 ,其测定的膜亲和性参数要明显比由疏水性参数预测的值高。结论　结合两性分子复杂的微观质子平衡 ,不仅其中性、阳性而且两性的微观离子可能通过匹配的构象和能量有利的作用模式而有效的分布到有序的两性磷脂膜中 ,并且后两者的分配产生了与有序磷脂膜的吸引性极性附加作用力 Objective To reveal the mode of action of amphiphile and ordered phospholipid membrane. Methods The membrane affinity and hydrophobicity parameters of the drug were determined by phospholipid membrane chromatography and n-octanol water respectively. Results The amphiphilic molecules and the ordered phospholipid membrane had attractive polar additive forces, and their measured membrane affinity parameters were significantly higher than those predicted by the hydrophobic parameter. Conclusions In combination with the complex microscopic proton balance of amphipathic molecules, not only is the neutral, positive, and amphoteric microscopic ions effectively distributed to the ordered amphiphilic phospholipid membrane through the matching conformational and energetic modes of action, and the distribution of the latter two An attractive additional polar force was generated with the ordered phospholipid membrane