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目的 检测感染HBV饮服人群的HBVDNA ,研究HBVDNA阳性者的家庭聚集性 ,探讨其传染性 ,为修订有关法规提供依据。 方法 用反向间接血凝法检测HBsAg ,用赖氏法检测ALT ,酶联免疫吸附试验检测HBVM ,聚合酶联反应 -微孔杂交法和荧光定量PCR法检测HBVDNA。对HBVDNA阳性者的家庭聚集性进行对照研究。 结果 在 4110 5名饮食服务人员中检出HBsAg阳性 14 82人 ,阳性率 3 .61%。在HBsAg <1:5 12、ALT <40、且HBeAg阴性的90 0人中检出HBVDNA阳性者 3 12人 ,阳性率 3 4.67%。HBVDNA阳性者的家庭聚集率为 86.49% ,家庭成员HBV感染率为 64 .3 8% ,相对危险性 6.5 8。HBVDNA阳性者的家庭成员HBV感染呈家庭聚集性。 结论 血清学检验结果同时表现为HBsAg <1:5 12、ALT <40、HBeAg阴性、但HBVDNA阳性的在岗饮食服务人员是HBV的危险传染源。
OBJECTIVE: To detect HBVDNA in HBV-infected subjects and to study the familial aggregation of HBVDNA-positive individuals and to explore their contagion, so as to provide the basis for the revision of relevant laws and regulations. Methods HBsAg was detected by reverse indirect hemagglutination, ALT by Lai’s method, HBVM by enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction-micropore hybridization and fluorescence quantitative PCR. A controlled study of familial aggregation in HBVDNA positive individuals. Results A total of 14 82 HBsAg positive cases were detected in 4,110 food service workers, with a positive rate of 3.61%. HBeAg <1: 5 12, ALT <40, and HBeAg-negative 90 0 were detected HBVDNA positive in 3 12, the positive rate of 3.67%. The family aggregation rate was 86.49% in HBVDNA positive patients and 64.38% in family members, with a relative risk of 6.58. HBV infection in family members with HBVDNA positive was family clustering. Conclusions The results of serological test also showed HBsAg <1: 5 12, ALT <40 and negative HBeAg, but HBVDNA positive on-the-job food service workers were the risk source of HBV infection.