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目的:以蛋白印记技术观察糖尿病大鼠坐骨神经中转录因子IκBa、Ikka的蛋白表达。方法:148只雄性Wistar大鼠,以STZ诱导糖尿病模型,随机分为正常组、模型组、辛味通络组、藤类通络组、虫类通络组。于治疗后4、8周处死大鼠取坐骨神经备蛋白印记观察用。结果:造模组IκBa、Ikka水平明显低于同期正常组(P<0.01),与同期模型组比较,通络中药组均能提高IκBa、Ikka的表达(P<0.01),同期治疗组之间无显著性差异。结论:不同通络中药随着疗程延长均具有延缓神经病变进程的作用,可能与调节IκBa、Ikka信号系统有关。
Objective: To observe the protein expression of IκBa and Ikka in the sciatic nerve of diabetic rats by Western blotting. Methods: One hundred and forty-eight male Wistar rats were randomly divided into normal group, model group, Xinwei Tongluo group, rattan and Tongluo group and insects Tongluo group. At 4 and 8 weeks after treatment, rats were sacrificed and the sciatic nerve was prepared for Western blotting. Results: The levels of IκBa and Ikka in model group were significantly lower than those in normal group (P <0.01). Compared with the model group, the expression of IκBa and Ikka in Tongluo Chinese herbs group was significantly increased (P <0.01) No significant difference. Conclusion: Different Tongluo herbs may delay the progression of neuropathic process with prolonged course of treatment, which may be related to the regulation of IκBa and Ikka signaling systems.