目的:从正常女性生殖道中分离乳杆菌,为开发复方益生菌制剂提供新型菌株。方法:采用传统方法鉴定出乳杆菌,进一步筛选出产生过氧化氢的优势菌株,并运用分子生物学方法,对其进行基因型鉴定。并对优势菌进行初步的性能鉴定试验。结果:从18名体检女性阴道分泌物内分离纯化得到11株菌,检出率为61.1%。分别为嗜酸乳杆菌4株,格氏乳杆菌3株,詹氏乳杆菌2株,卷曲、唾液乳杆菌各1株。结论:运用传统的细菌学分离方法与分子生物学对分离的菌株进行了鉴定。其中一株为产生过氧化氢的卷曲乳杆菌,经过初步测试,该菌株具有良好的耐酸、耐高温及体外拮抗其他病原微生物的能力。 OBJECTIVE: To isolate Lactobacillus from the normal female reproductive tract and to provide a novel strain for the development of compound probiotics. Methods: Lactobacillus was identified by the traditional method, and the dominant strains producing hydrogen peroxide were further screened, and their genotypes were identified by molecular biology method. The dominant bacteria were tested for their performance. Results: Eleven strains were isolated and purified from 18 female vaginal secretions. The detection rate was 61.1%. They were 4 strains of Lactobacillus acidophilus, 3 strains of Lactobacillus gasseri, 2 strains of Lactobacillus jinnii, 1 strain of curly and Lactobacillus salivarius respectively. Conclusion: The isolated strains were identified by traditional bacteriological methods and molecular biology. One of the strains is hydrogen peroxide-producing Lactobacillus curvatus. After preliminary tests, the strain has good acid-fastness, high temperature resistance and antagonism of other pathogenic microorganisms in vitro.