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以玉米自交系齐319(Qi319)为试材,利用叶绿素荧光快速诱导动力学曲线,研究了喷施外源激素6-苄氨基嘌呤(6-BA)对不同氮素水平下玉米花后叶片光系统II(PSII)性能的调控效应。两年研究结果表明,花后穗位叶叶绿素含量和净光合速率(Pn)均呈降低趋势。在不施氮条件下,外源喷施6-BA,可提高叶绿素含量及后期Pn,但对PSII反应中心的活性无显著改善。施氮可显著提高Pn和PSII的性能,6-BA可显著增强其效果。二者互作条件下,叶绿素含量显著提高,Pn显著增加(P<0.05),PSII供体侧和受体侧的活性得到有效改善,其中对PSII供体侧性能的改善幅度大于对受体侧,花后10dPSII反应中心的活性提高最显著。光合性能的提高使单株生物量和籽粒产量显著增加(P<0.05)。因此,在适量施用氮肥的条件下结合喷施6-BA可以显著改善叶片光合性能。
Using maize inbred line Qi 319 (Qi319) as test material, the rapid kinetics curve of chlorophyll fluorescence was used to study the effects of spraying 6-benzylaminopurine (6-BA) Regulation of PSII performance. The results of two years showed that the chlorophyll content and net photosynthetic rate (Pn) of the ear leaves showed a decreasing trend. Under the condition of no nitrogen application, 6-BA exogenous spraying could increase the content of chlorophyll and increase the content of Pn in the late stage, but did not significantly improve the activity of PSII reaction center. Nitrogen application can significantly improve the performance of Pn and PSII, 6-BA can significantly enhance its effect. Under the interaction of the two, the content of chlorophyll increased significantly, Pn increased significantly (P <0.05), and the activity of donor and acceptors of PSII was improved effectively. The improvement of PSII donor side performance was more than that of donor side , Post-flow 10dPSII reaction center to enhance the most significant activity. The improvement of photosynthetic characteristics significantly increased the biomass per plant and the grain yield (P <0.05). Therefore, spraying 6-BA in combination with nitrogen fertilizer could significantly improve leaf photosynthesis.