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目的:研究痛泻要方对肠易激综合征(IBS)大鼠内脏高敏性外周机制的作用。方法:于乳鼠第8-21天以血管成形术球囊/儿童导尿管插入直肠,并向球囊注水以扩张肠道刺激,2次/d,每次持续1min制备IBS内脏高敏性大鼠模型。分别于直肠扩张刺激停止后2周及4周后进行肠道敏感性评估筛选成模大鼠。大鼠出生第12周,IBS内脏高敏性大鼠分别灌胃痛泻要方2.03g/kg,给药2周。第14周分别取各组大鼠结肠组织进行组织病理学观察;以RT-PCR和免疫组化染色法分析结肠组织中5-羟色胺4受体(5-HT4)mRNA、c-fos mRNA及胃肠激素中生长抑素(SS)、血管活性肠肽(VIP)蛋白的表达变化。结果:经乳鼠结肠刺激方法复制内脏高敏性模型后,肠道敏感性有不同程度增强,然而结肠组织病理学无明显变化,结肠中5-HT4 mRNA表达降低,c-fos mRNA及SS、VIP表达增强,经痛泻要方干预后,肠道敏感性明显降低,5-HT4受体mRNA表达增强,c-fos mRNA及SS、VIP表达均明显降低(P<0.01)。结论:痛泻要方可通过调控外周肠神经丛传入神经元敏感性而发挥抑制内脏高敏性作用。
Objective: To study the effect of Tongxieyaofang on visceral hypersensitivity peripheral mechanism in irritable bowel syndrome (IBS) rats. Methods: The neonatal rats were inserted into the rectum with catheterization of angioplasty balloon / children for 8-21 days and infused into the balloon to dilate intestinal stimulation for 2 times / d, each time lasting for 1 min to prepare IBS visceral hypersensitivity Mouse model. Intestinal sensory evaluation was performed at 2 and 4 weeks after cessation of rectal dilatation to screen rats. At the 12th week of birth, IBS visceral hypersensitivity rats were intragastrically given Tongxieya prescription 2.03g / kg for 2 weeks. At the 14th week, the colon tissues of the rats in each group were observed histopathologically. The expression of 5-HT4 mRNA and c-fos mRNA in the colon tissues was analyzed by RT-PCR and immunohistochemistry Changes of the expressions of somatostatin (SS) and vasoactive intestinal peptide (VIP) in gut hormones. Results: The colon sensitivity was increased to some extent in the rat model of visceral hypersensitivity. However, the histopathology of colon did not change significantly. The expression of 5-HT4 mRNA in the colon decreased. The c-fos mRNA and SS, VIP The expression of 5-HT4 receptor mRNA was significantly increased, while the expression of c-fos mRNA, SS and VIP were significantly decreased (P <0.01). Conclusion: Tongxieyingfang can inhibit visceral hypersensitivity by regulating the sensitization of peripheral neurons to afferent neurons.