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应用正交表L_(18)(6~1×3~6)对KNO_3、NH_4NO_3、KH_2PO_4、MgSO_4·7H_2O、CaCl_2·2H_2O、铁盐和微量元素的不同水平进行试验,筛选出C_(17)培养基。在7个微量元素试验中,其结果是去掉NaMoO_4·2H_2O和将MnSO_4·4H_2O降为11.2毫克/升较好。 5种有机物中以D-生物素(维生素H)对花药愈伤组织的诱导频率影响最大,以1.5毫克/升最好。1980年河北省农科院粮油作物研究所C_(17)培养基的出愈率达12.19%。花药愈伤组织的质量好,分化率最高达50%。
The different levels of KNO_3, NH_4NO_3, KH_2PO_4, MgSO_4 · 7H_2O, CaCl_2 · 2H_2O, iron salts and trace elements were tested by orthogonal test table L_ (18) (6 ~ 1 × 3 ~ base. In the seven trace element tests, the result is that it is better to remove NaMoO 4 · 2H 2 O and reduce MnSO 4 · 4H 2 O to 11.2 mg / L. Of the five organic compounds, D-biotin (vitamin H) had the greatest effect on the induction frequency of anther callus, with 1.5 mg / l being the best. In 1980, Hebei Academy of Agricultural Sciences Institute of grain and oil crops C_ (17) medium out of 12.19%. Anther callus quality, differentiation rate up to 50%.