论文部分内容阅读
日前,中国农业科学院植物保护研究所研发的“一种罹病植物组织中香蕉穿孔线虫DNA提取液、提取方法及其应用”的新技术获得国家发明专利,其专利号为ZL201110340265.2。该发明涉及植物病害的分子生物学检测技术。所述DNA提取液其组分及各组分的终浓度如下:Tris-HCl200mM,PH 8.0;NaCl 400mM;EDTA 10mM,PH8.0;CTAB 3%(W/V);β-巯基乙醇4%(V/V)。该提取液作为罹病植物香蕉穿孔线虫DNA的裂解液,能够将感染植物组
Recently, the Institute of Plant Protection, Chinese Academy of Agricultural Sciences developed a “disease-ridden plant tissues in the DNA extract of banana perforation nematodes, extraction methods and applications ” new technology access to national invention patents, the patent number is ZL201110340265.2. This invention relates to the molecular biology detection of plant diseases. The final concentrations of the components and the components of the DNA extract are as follows: Tris-HCl 200 mM, pH 8.0; NaCl 400 mM; EDTA 10 mM, pH 8.0; CTAB 3% (W / V); β-mercaptoethanol 4% V / V). The extract as a lyophilized plant Banana perforated nematode DNA lysis solution can be infected plant group