论文部分内容阅读
以短枝富士 (SpurFuji)×舞姿 (Telamon)的 10 5株F1群体为试材 ,利用RAPD技术 ,结合集群分类分析法(BSA)进行了苹果柱型基因 (Co)分子标记的研究。通过对 30 0条随机引物的筛选 ,获得一个与Co基因紧密连锁的RAPD标记S114 2 682 ,连锁距离为 2 .86cM。对该标记片段进行序列测定 ,然后根据序列特点设计了 4条特异引物(其中正向引物与反向引物各两条 )。PCR结果显示 ,这 4条引物的 4种组合都可以扩增出柱型性状的特征带。选其中之一进行群体上的分析 ,结果表明该SCAR标记特征带与柱型性状的共分离行为与原RAPD标记表现一致。可见 ,此组合的引物可以作为该SCAR标记的特异引物。通过对S114 2 682 标记片段序列分析发现 ,在 +45～ +2 5 1区域含有一个可编码 6 8个氨基酸残基的ORF。
A total of 105 F1 populations of Spurfuji × Telamon were used as materials to study the molecular marker of apple (Co) gene by RAPD and cluster analysis (BSA). By screening 30 random primers, a RAPD marker S114 2 682 closely linked to the Co gene was obtained, with a linkage distance of 2.86 cM. The sequence of the fragment was sequenced, and then four specific primers were designed according to the sequence characteristics (two of them were forward and reverse). PCR results showed that all four combinations of these four primers could amplify the characteristic bands of column traits. One of them was selected for population analysis. The results showed that the co-segregation behavior between the SCAR marker characteristic band and the trait trait was consistent with the original RAPD marker. It can be seen that the primer of the combination can be used as a specific primer of the SCAR marker. Sequence analysis of S114 2 682 showed that there was an ORF encoding 68 amino acid residues in the region of +45 ~ +2 51.