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用ＤＣＡ—Ｎａ，诱发大鼠急性胰腺炎（ＡｃｕｔｅＰａｎｃｒｅａｔｉｔｉｓ），探讨ＡＰ的发病机制；对比茵陈蒿汤及其组分对胰腺腺泡溶酶体膜稳定性的影响。按析因法设计实验。用差速离心技术，以２５０００ｇ３０分钟分离溶酶体。测上清及沉淀中溶酶体标记酶—酸性磷酸酶（ＡＣＰ）的活性，以总酶活比作为溶酶体膜稳定性的主要指标。结果显示：各中药治疗组沉淀溶酶体ＡＣＰ活性较盐水组高，而总酶活比显著降低，接近正常组。提示：ＡＰ时胰腺腺泡溶酶体膜稳定性降低，而茵陈蒿汤及其组分对溶酶体膜有保护作用。
DCA-Na was used to induce rat acute pancreatitis (Acute Pancreatitis) and the pathogenesis of AP was investigated. The effect of Yinchenhao decoction and its components on the stability of lysosome membrane of pancreatic acinar was compared. According to the factorial design experiments. Lysosomes were separated at 25000g for 30 minutes using differential centrifugation techniques. The activity of lysosomal labeling enzyme, acid phosphatase (ACP), was determined in supernatants and sediments, and the total enzyme activity ratio was used as the main indicator of lysosome membrane stability. The results showed that the activity of ACP of precipitated lysosomes in the traditional Chinese medicine treatment group was higher than that in the saline group, and the total enzyme activity ratio was significantly decreased, which was close to the normal group. It is suggested that the stability of pancreatic acinar lysosomal membranes is decreased in AP, while the Yinchenhao decoction and its components have protective effects on lysosomal membranes.