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目的比较常规IVF授精后4h和20h补行卵胞浆内单精子注射(rescue ICSI)的效果。方法回顾分析2006年1月-2007年12月在我院生殖医学中心接受常规IVF治疗的21例非男性因素不育患者,授精后4h行补救ICSI,并与另组实施的18个常规IVF授精后20h行补救ICSI周期进行比较。结果 4h补救ICSI组的受精率为76.3%,高于20h补救ICSI组(53.0%),有统计学意义(P<0.05)。4h补救ICSI组中11例临床妊娠(52.3%),其中2个双胎,8个单胎和一个流产。而20h补救ICSI组无一例妊娠。结论和授精后20h行补救ICSI相比,授精后4h行补救ICSI可以获得较好的受精率、妊娠率和种植率。
Objective To compare the effect of intracytoplasmic resuscitation ICSI 4h and 20h after IVF fertilization. Methods A retrospective analysis of 21 non-male infertility patients who received routine IVF treatment at the Reproductive Medicine Center of our hospital from January 2006 to December 2007 was performed. ICSI was rehabilitated 4 hours after fertilization and 18 routine IVF insemination After 20h ICSI cycle recovery for comparison. Results The rate of fertilization in ICSI group was 76.3% at 4h, and was higher than that in ICSI group at 20h (53.0%) (P <0.05). In the ICH group, 11 clinical pregnancies (52.3%) were recovered in 4 h, of which 2 were twins, 8 were singletons and one was aborted. 20h recovery ICSI group without a case of pregnancy. Conclusion Compared with ICSI at 20h after fertilization, ICSI at 4h after fertilization can achieve better fertilization rate, pregnancy rate and implantation rate.