论文部分内容阅读
应用反相高效液相色谱法对口灌和肌肉注射氟苯尼考在欧洲鳗鲡(Anguilla anguilla)体内的代谢规律进行了研究。按100mg.kg-1口灌给药后血浆、肌肉、肝脏、肾脏中氟苯尼考浓度的达峰时间分别为2h、6h、0.5h、1h,以后开始缓慢下降,给药2d后血浆、肌肉、肝脏、肾脏中的氟苯尼考浓度分别为4.209μg.mL-1、0.792μg.g-1、0.493μg.g-1、1.448μg.g-1,给药3d后血浆中的氟苯尼考浓度分别为0.0836μg.mL-1,肌肉、肝脏、肾脏中的氟苯尼考浓度均未检出;按100mg.kg-1肌肉注射给药后血浆中氟苯尼考浓度达峰时间为0.5h,以后开始缓慢下降,给药5d后血浆中的氟苯尼考浓度为0.1151μg.mL-1,给药10d后血浆中的氟苯尼考浓度未检出。口灌氟苯尼考在欧洲鳗鲡体内血浆、肌肉、肝脏、肾脏中分布可用开放性二室模型来描述,口灌给药的血浆、肌肉、肝脏、肾脏中的消除半衰期(T1/2β)分别为27.939h、18.844h、11.83h、36.87h;肌肉注射氟苯尼考在欧洲鳗鲡体内血浆、肌肉、肝脏、肾脏中分布可用开放性一室模型来描述,肌肉注射给药的血浆中的消除半衰期(T1/2β)为37.52h。
The application of florfenicol in oral and intramuscular injection of Anguilla anguilla was studied by reversed-phase high performance liquid chromatography (RP-HPLC). The peak time of florfenicol in plasma, muscle, liver and kidney was 2h, 6h, 0.5h, 1h respectively after oral administration of 100mg.kg-1, and then decreased slowly. After 2d administration, plasma, The concentrations of florfenicol in muscle, liver and kidney were 4.209μg.mL-1, 0.792μg.g-1, 0.493μg.g-1, and 1.448μg.g-1, respectively. Benzene concentration of 0.0836μg.mL-1, muscle, liver, kidney florfenicol concentrations were not detected; according to 100mg.kg-1 intramuscular injection of fofenilol plasma concentration peak The time began to decline slowly. After 5 days of administration, the concentration of florfenicol in plasma was 0.1151μg.mL-1, and the concentration of florfenicol in plasma was not detected after 10 days. The oral florfenicol distribution in plasma, muscle, liver and kidney of European eel can be described by an open two-compartment model. The elimination half-life (T1 / 2β) in oral administration of plasma, muscle, liver and kidney 27.939h, 18.844h, 11.83h and 36.87h, respectively. The distribution of florfenicol intramuscularly in the plasma, muscle, liver and kidney of European eel can be described by an open-studio model. The elimination of intramuscular injection of plasma The half-life (T1 / 2β) was 37.52h.