论文部分内容阅读
目的:观察中国汉族和高加索糖尿病肾病(DN)患者肾小球内差异表达基因和发生调节的分子信号通路的异同。方法:分别选取中国汉族DN患者29例和正常对照5例,高加索DN患者21例及正常对照35例,微分离肾小球进行全基因组数据表达检测,分析不同人种DN患者与正常对照相比出现差异表达的基因;分析两个人种DN患者肾小球内与估算的肾小球滤过率(eGFR)变化相关的表达基因;比较差异表达基因与eGFR变化相关基因在两个人群中的异同。结果:(1)中国汉族DN患者肾小球中有565个基因存在差异表达,高加索DN患者肾小球中有775个基因存在差异表达;(2)比较分析提示,有200个基因在两个人种均存在差异表达。分子通路分析提示,共有13条信号通路在两个人种中均发生调节,其中大部分为免疫炎症反应相关性通路;(3)中国汉族人群中有225个基因与eGFR明显相关;而在高加索人群中有293个基因与eGFR明显相关。比较分析提示,共有CRISPLD2、SPON1、LUM等55个基因在两个人种中均与eGFR水平明显相关。结论:通过比较分析中国汉族人群和高加索人群DN患者肾小球全基因组表达谱的异同发现,免疫炎症通路在不同人种DN的发生发展中均发挥重要作用。同时,我们还证实了55个与DN患者肾功能改变明显相关的基因,可作为判断DN患者疾病进展分子标志物的候选基因进行研究验证。
OBJECTIVE: To observe the similarities and differences between differentially expressed genes in glomeruli and molecular signaling pathways in patients with diabetic nephropathy (DN) of Chinese Han and Caucasians. Methods: Totally 29 Chinese Han patients with DN, 5 normal controls, 21 normal Caucasian patients with DN and 35 normal controls were enrolled in this study. Genomic data were detected by differential nephrolithiasis and compared with normal controls Differentially expressed genes; analysis of glomerular glomerular expression of genes associated with estimated glomerular filtration rate (eGFR) changes in two human DN patients; comparison of similarities and differences between the differentially expressed genes and eGFR-related genes in two populations . Results: (1) 565 genes were differentially expressed in glomeruli of Chinese Han patients with DN, and 775 genes were differentially expressed in glomeruli of Caucasian DN patients. (2) Comparative analysis indicated that there were 200 genes in two There are differences in species are expressed. Molecular pathway analysis suggested that a total of 13 signaling pathways were regulated in both populations, most of which were immune-inflammatory response-related pathways; (3) 225 Chinese Han population were significantly associated with eGFR; whereas in Caucasians 293 genes were significantly associated with eGFR. Comparative analysis suggests that 55 genes, including CRISPLD2, SPON1, and LUM, are significantly associated with eGFR levels in both populations. CONCLUSION: Through the comparative analysis of the whole genome expression profiles of glomeruli in patients with DN in Chinese Han and Caucasian population, it is found that immune and inflammatory pathways play an important role in the occurrence and development of DN in different ethnic groups. At the same time, we also confirmed that 55 genes that were significantly associated with renal function changes in patients with DN could be used as candidate genes for molecular markers of disease progression in DN patients.