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氧化应激是由活性氧自由基生产量增加或代谢量减少而产生积聚,进而引细胞凋亡的原因。综述细胞氧化应激检测方法,筛选适合检测神经细胞的检测方法:JC-1染色检测细胞线粒体膜电位(MMP)、ELISA法检测超氧化物歧化酶(SOD)、Western bloting法检测GRP78蛋白。
Oxidative stress is caused by the increased production of reactive oxygen species (ROS) or decreased metabolism, which leads to the accumulation of apoptotic cells. The detection methods of cell oxidative stress were summarized, and the suitable detection methods of neural cells were screened: JC-1 staining was used to detect the mitochondrial membrane potential (MMP), superoxide dismutase (SOD) by ELISA and GRP78 protein by Western blotting.