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作者用人株(BAH54)、波特兰株(PI)及其克隆株(PIC10)3株蓝氏贾第鞭毛虫滋养体进行阿苯达唑抗贾第虫体外实验,并与甲硝唑和替硝唑进行比较。甲硝唑和替硝唑用双蒸水稀释,阿苯达唑用二甲基亚砜(DMSO)稀释,试验浓度分别为0.1、0.5、1.0、2.0及10.0μg/ml等。受试虫株均培养在含有10%新生小牛血清,而不含抗生素与维生素混合液的BI-S-33培养基中,虫体浓度范围为30000～50000个滋养体/ml,在培养基中加入适当浓度的药后,37℃培养。药物处理的时间分别为4h和24h,然后将虫体置换于无药培养基中培养到7天,另设双蒸水或DMSO的无药对照组,用半定量法测定滋养体的吸附能力。与对照组相比较,对滋养体形态、增
In this study, the in vitro experiments of albendazole against Giardia lamblia were conducted in vitro with three G. gondii trophozoites from human strain (BAH54), Portland strain (PI) and its clone (PIC10) Nitrex compared. Metronidazole and tinidazole diluted with double distilled water, albendazole with dimethyl sulfoxide (DMSO) dilution, the test concentrations were 0.1,0.5,1.0,2.0 and 10.0μg / ml and so on. The tested strains were cultured in BI-S-33 medium containing 10% of newborn calf serum but without a mixture of antibiotics and vitamins at concentrations ranging from 30,000 to 50,000 trophozoites / ml in culture medium After adding the appropriate concentration of drug, 37 ℃ culture. Drug treatment time were 4h and 24h, respectively, and then the parasites were replaced in non-drug-resistant culture medium to 7 days, another set of double distilled water or DMSO non-drug control group, semi-quantitative determination of trophozoite adsorption capacity. Compared with the control group, the trophozoite morphology increased