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在水/有机溶剂双相反应体系中,研究了来源于西梅的(R)-醇腈酶催化酮与丙酮醇腈合成(R)-酮醇腈的立体选择性转氰反应.系统探讨了不同酶源、酶粉颗粒大小、底物浓度、两底物配比、酶浓度和底物结构对转氰反应的影响.结果发现西梅醇腈酶能高效催化三甲基硅酮与丙酮醇腈的立体选择性转氰.酶粉颗粒大小以直径0.3～0.45mm为优,底物浓度以21mmol/L左右为佳,底物丙酮醇腈与三甲基硅酮摩尔浓度比以2∶1为宜,酶浓度以60.9g/L左右为好.西梅醇腈酶对3,3-二甲基-2-丁酮几乎没有催化活性,而对其硅结构类似物三甲基硅酮却具有非常高的立体选择性和催化活性,在上述优化反应条件下反应24h的底物转化率和产物光学纯度均高达99%以上,表明底物中的硅原子对西梅醇腈酶的催化活性有非常显著的促进作用.
In the water / organic solvent biphasic reaction system, the (R) -hydroxycarbonitrile enzyme derived from prune was used to catalyze the stereoselective cyano- The effects of different enzyme sources, enzyme particle size, substrate concentration, the ratio of two substrates, the enzyme concentration and the substrate structure on the reaction of cyanogen conversion were investigated. The results showed that the reaction of trimethylolpropane trimethoxysilane with acetol Nitrile three-dimensional selective cyanogen. Enzyme powder particle size of 0.3 ~ 0.45mm diameter is better, the substrate concentration of about 21mmol / L is better substrate molar ratio of acetone and nitrile trimethylsilyl 2: 1 Is appropriate, the enzyme concentration of about 60.9g / L. Good plum-cyanoacrylate on 3,3-dimethyl-2-butanone has almost no catalytic activity, and its analogues of silicon structure but trimethylsilicone Has a very high stereoselectivity and catalytic activity, under the optimal reaction conditions 24h reaction substrate conversion and the optical purity of the product were as high as 99%, indicating that the silicon atoms in the substrate of the reaction of cimetidolnitrile enzyme activity There is a very significant role in promoting.