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用原位杂交法检测小鼠不同培养时期的ＴＩＬ和ＬＡＫ细胞中穿孔蛋白（ＰＦＰ）ｍＲＮＡ的表达，并用显微图象分析系统处理检测ＰＦＰｍＲＮＡ表达水平，结果表明：ＴＩＬ细胞的ＰＦＰｍＲＮＡ表达水平在第１４、２１天时达高峰，２８天时开始下降；而ＬＡＫ细胞则在第７天时即达到较高水平，２１天时开始下降，但ＴＩＬ细胞的表达水平显著高于ＬＡＫ。将同时期的ＴＩＬ及ＬＡＫ细胞进行体内外抗瘤活性测定，发现两者的ＰＦＰｍＲＮＡ表达水平，均与其体外杀伤活性有明显的相关性，而体内抗瘤活性与体外杀伤活性间则无显著相关性。研究结果提示，ＰＦＰ在小鼠ＴＩＬ，ＬＡＫ细胞介导的溶细胞过程中起着重要作用，而ＴＩＬ和ＬＡＫ细胞的体内抗瘤活性还受体内多种因素的影响。
The in situ hybridization was used to detect the expression of PFP mRNA in TIL and LAK cells of different culture stages. The expression of PFP mRNA was detected by microscopic image analysis system. The results showed that the expression level of PFP mRNA in TIL cells was Peaked on day 21 and reached its peak on day 28, and began to decline on day 28. LAK cells reached a high level on the 7th day and began to decline on the 21st day, but the level of TIL cells was significantly higher than that on the LAK. TIL and LAK cells in the same period of in vitro and in vivo anti-tumor activity assay and found that the two PFPmRNA expression levels were in vitro cytotoxicity was significantly correlated with the in vivo anti-tumor activity and in vitro anti-tumor activity was no significant correlation . The results suggest that PFP plays an important role in the cytolytic process mediated by TIL and LAK in mice, while the antitumor activity of TIL and LAK cells in vivo is also influenced by many factors in the body.