测定生物体内巯甲丙脯酸已有GC-MS、GC及HPLC方法,但这些方法费时、繁琐。本文介绍一种灵敏、特异的酶免疫分析法。仪器与试剂:MPF-4型荧光分光光度计、LSC-900型液体闪烁计数器、离心机、恒温器、13×100mm玻璃管。氚化巯甲丙脯酸乙酸酯、N-(3-二甲胺丙基)-N′-乙基碳化二亚胺盐酸盐(DEC)、牛免疫球蛋白G(BIgG)、牛血清白蛋白(BSA)、02-D-半乳糖苷酶、免疫珠第二抗体、山羊抗兔免疫球蛋白 GC-MS, GC and HPLC methods have been developed for the determination of captopril in vivo, but these methods are time consuming and cumbersome. This article describes a sensitive and specific enzyme immunoassay. Instruments and reagents: MPF-4 fluorescence spectrophotometer, LSC-900 liquid scintillation counter, centrifuge, thermostat, 13 × 100mm glass tube. Tritiated captopril acetate, N- (3-dimethylaminopropyl) -N’-ethylcarbodiimide hydrochloride (DEC), bovine immunoglobulin G (BIgG), bovine serum Albumin (BSA), 02-D-galactosidase, immunobeads secondary antibody, goat anti-rabbit immunoglobulin