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目的:探讨腺苷A2A受体、多巴胺D2受体及纹状体-苍白球通路在运动疲劳调控中的作用。方法:Wistar雄性健康大鼠30只,随机分为3组:运动对照组(CG)、腺苷A2A受体拮抗剂干预组和多巴胺D2受体激动剂干预组,每组10只。3组大鼠从纹状体分别微量注射人工脑脊液、特异性腺苷A2AR拮抗剂SCH58261和D2DR激动剂喹吡罗,采用金属微电极分别记录1次力竭运动过程中大鼠同侧苍白球局部场电位的动态变化,结合运动能力进行观察并记录力竭运动时间。结果:苍白球神经元电活动在力竭运动不同阶段出现明显变化特征,相比安静状态,力竭即刻神经元兴奋性显著下降,β波的PSD值增加(P<0.05),γ波PSD值显著下降(P<0.05),功率谱重心频率降至最低(P<0.05)。A2AR拮抗剂和D2DR激动剂干预能够改变相应波段的振幅,在运动开始75 min、力竭即刻与恢复期,均显著高于CG(P<0.05),延缓重心频率的下降和大鼠跑台运动至力竭的时间。结论:1次力竭运动过程中大鼠苍白球神经元局部场电位随着运动强度和时间的增加而呈现阶段性变化特征,主要体现为出现明显的振荡活动(β波和γ波振荡),且β波和γ波之间的转换和功率的变化与运动状态有关;苍白球神经元的兴奋性下降可能是导致运动疲劳产生的中枢机制之一,证明苍白球参与运动疲劳的中枢调控;SCH58261和喹吡罗经纹状体微注射后,可以通过各自受体抑制力竭时D2-MSNs的过度兴奋,调节β波和γ波频段的振幅,从而避免苍白球神经元兴奋性下降过快,降低苍白球通过皮层-基底神经节环路对皮层的抑制作用,延缓疲劳发生。
AIM: To investigate the role of adenosine A2A receptor, dopamine D2 receptor and striatum-globus pallidus in the regulation of sports fatigue. Methods: Thirty male Wistar rats were randomly divided into 3 groups: exercise control group (CG), adenosine A2A receptor antagonist intervention group and dopamine D2 receptor agonist intervention group (n = 10). Three groups of rats were injected with artificial cerebrospinal fluid, SCH58261, a specific adenosine A2AR antagonist, and quinpirole, a D2DR agonist respectively, from the striatum. One metal microparticle was used to record the local field of ipsilateral globus pallidus during exhaustive exercise Potential changes, combined with exercise capacity to observe and record exhaustion exercise time. Results: Compared with resting state, the electrical activity of globus pallidus neurons showed obvious changes at different stages of exhaustive exercise. The excitability of neurons in exhaustive group decreased significantly (P <0.05) and the value of PSD (P <0.05), and the frequency of the center of gravity of the power spectrum decreased to the lowest (P <0.05). A2AR antagonist and D2DR agonist could change the amplitude of the corresponding wave band at 75 min after start of exercise, immediately after exhaustive exercise and recovery period, significantly higher than that of CG (P <0.05), delayed the decrease of center of gravity frequency and treadmill running To exhaust time. CONCLUSION: The local field potentials of pallidocytes in rats during one exhaustive exercise show the characteristics of periodic changes with the increase of exercise intensity and time, which are mainly manifested by significant oscillations (β wave and γ wave oscillation) And the change of β-wave and γ-wave and the change of power are related to the state of motion. The decrease of excitability of globus pallidus neurons may be one of the central mechanisms leading to exercise fatigue, demonstrating that globus pallidus participates in the central regulation of exercise-induced fatigue. SCH58261 And quinpirole via microinjection of the striatum can inhibit the over-excitement of exhausted D2-MSNs through their respective receptors and modulate the amplitude of the beta and gamma wave bands, thereby preventing the decrease of the excitability of the globus pallidus neurons Globus palliation through the cortex - basal ganglia loop inhibition of the cortex, delay fatigue.