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本研究用纯化Ｅ．ｃｏｌｉＲｅｃＡ蛋白免疫ＢＡＬＢ／ｃ小鼠，取免疫脾细胞与小鼠骨髓瘤细胞Ｓｐ２／０融合，以粗制Ｅ．ｃｏｌｉＲｅｃＡ包被酶标反应板，间接ＥＬＩＳＡ方法筛选阳性孔，经有限稀释法４次克隆化，建立了两株分泌抗ＲｅｃＡ蛋白ＭｃＡｂ的细胞系２Ｄ６和３Ｂ１。免疫印迹表明ＭｃＡｂ只对细菌的ＲｅｃＡ有反应，不与细菌其它成分发生反应。间接ＥＬＩＳＡ进一步表明，ＭｃＡｂ２Ｄ６、３Ｄ１只对细菌ＲｅｃＡ类蛋白有反应，不与小鼠肝、心、肺、脾等和ＢＨＫ细胞的内外蛋白反应。
This study used purified E. The BALB / c mice were immunized with the coliRecA protein, and the splenocytes were fused with Sp2 / 0 of mouse myeloma cells. The positive clones were screened by indirect enzyme-linked immunosorbent assay (ELISA) and cloned by restriction endonuclease four times. Two cell lines, 2D6 and 3B1, secreting anti-RecA McAb were established. Western blotting showed that McAb reacted only to bacterial RecA and did not react with other bacterial components. Indirect ELISA further showed that McAb2D6,3D1 only reacted with bacterial RecA protein and did not react with the internal and external proteins of mouse liver, heart, lung, spleen and other BHK cells.