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A novel method based on plasma polymerized films(PPFs) is proposed for immobilization of antibodies(antigens). PPFs are prepared on the surfaces of base sensors by a ratio frequency plasma method with n butyl amine as a plasma source, and the IR spectrum indicates the existence of amino group in the film. A polyanion layer is then self assembled on the film through strong electrostatic attraction. Subsequently, antibodies(antigens) are immobilized on the self assembled layer through controlling the pH value of immobilizing solution. The immobilization of goat anti IgG antibody, as an example, on the surfaces of quartz crystal microbalance (QCM) is investigated. After depositing a n butyl amine PPF on the surface of QCM, a polystyrenesulfonate (PSS) layer is self assembled on the PPF from a 2 0 mg/mL of PSS solution in 0 05 mol/L HCl. The goat anti IgG antibody is then immobilized in antibody immobolizing solution(0 2 mg/mL goat anti IgG antibody, pH 5 0). This immobilization shows a better frequency response than the covalent immobilization with glutaraldehyde. The QCM immunosensor can quantitatively be used to determine NHIgG in the range of 0.7~126 μg·mL -1 , and shows a good reproducibility. Moreover, the immunosensor can be easily regenerated by using a pH shift.
A novel method based on plasma polymerized films (PPFs) is proposed for immobilization of antibodies (antigens). PPFs are prepared on the surfaces of base sensors by a ratio frequency plasma method with n butyl amine as a plasma source, and the IR spectrum indicates the existence of amino group in the film. A immobilization on the film through strong electrostatic attraction. antibodies (antigens) are immobilized on the self assembled layer through controlling the pH value of immobilizing solution. anti-IgG antibody, as an example, on the surfaces of quartz crystal microbalance (QCM) was investigated. After depositing an butyl amine PPF on the surface of QCM, a polystyrenesulfonate (PSS) layer is self assembled on the PPF from a 2 0 mg / mL of PSS solution in 0 05 mol / L HCl. The goat anti IgG antibody is then immobilized in an antibody immobilization solution (0 2 mg / mL goat anti IgG antibody, pH 50). immobilization shows a better frequency response than the covalent immobilization with glutaraldehyde. The QCM immunosensor can be quantitatively be used to determine NHIgG in the range of 0.7 to 126 μg · mL -1, and shows a good reproducibility. by using a pH shift.