论文部分内容阅读
目的:研究谷氨酸(Glu)对大鼠脂肪干细胞Adipose-derived stem cells(ADCSs)诱导分化神经细胞的作用及机制。方法:取成年大鼠腹股沟脂肪组织进行体外细胞培养,采用免疫组化方法检测证实为ADSCs。对照组为正常培养的ADSCs并诱导分化神经细胞,谷氨酸(Glu)处理组加入不同浓度的Glu,MTT比色法观察脂肪干细胞的存活率。结果:从ADSCs诱导分化的细胞包括神经元及神经胶质细胞,免疫组化结果显示神经元特异性烯醇化酶(NSE)染色阳性和胶质纤维酸性蛋白(GFAP)染色阳性。Glu处理组给药24h后,与对照组比较ADSCs存活率明显降低,50μmol·L-1Glu组细胞存活率为83.98%,与对照组比较差异无显著性(P>0.05);100及1000μmol·L-1Glu组干细胞存活率(分别为66.82%和17.08%)低于对照组(P<0.05,P<0.01)。结论:Glu对ADSCs有损伤作用,随着Glu剂量的增加,ADSCs的存活率逐渐降低,二者呈剂量依赖关系。
AIM: To investigate the effect of glutamate (Glu) on differentiating neural cells induced by Adipose-derived stem cells (ADSCs) in rat adipose derived stem cells (ADSCs) and its mechanism. Methods: Adult rat inguinal adipose tissue cells were cultured in vitro and confirmed by immunohistochemistry as ADSCs. The control group was normal cultured ADSCs and induced differentiation of neural cells, Glu concentration of different concentrations of Glu, MTT colorimetric assay of adipose-derived stem cell survival rate. Results: The cells differentiated from ADSCs included neurons and glial cells. The results of immunohistochemistry showed that neuron specific enolase (NSE) staining and glial fibrillary acidic protein (GFAP) staining were positive. Compared with the control group, the survival rate of ADSCs in Glu treatment group was significantly lower than that in the control group at 24 hours after treatment with Glu. The cell survival rate was 83.98% in the 50μmol·L-1Glu group, with no significant difference compared with the control group (P> 0.05); 100 and 1000μmol·L The survival rate of stem cells in -1Glu group (66.82% and 17.08%, respectively) was lower than that in control group (P <0.05, P <0.01). Conclusion: Glu has a damaging effect on ADSCs. With the increase of Glu dose, the survival rate of ADSCs gradually decreases, which shows a dose-dependent relationship.